1. Paste your sequence here: (Fasta, SwissProt or Plain Text format)  
2. Select one of the following: You want to specify the transmembrane segment(s) yourself (using drop down menus). Choose the number of transmembrane segments to use from this menu: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 After pressing 'continue' you will be prompted to select the endpoints for each segment.
Your sequence is in SwissProt format and you want to use the 'FT TRANSMEM' keys in it.
3.