Appendix to paper: A novel method for predicting transmembrane segment in proteins based on a statistical analysis of the SwissProt database: the PRED-TMR algorithm

Table 1: Set of 101 non homologous proteins used as a test set for the PRED-TMR algorithm. These were chosen from a set of 155 proteins (Table 2, below) collected from sequences used in three recent papers (Rost et al., 1995; Rost et al., 1996; Cserzo et al., 1997), discarding those with more than 25% homology. The 64 proteins used for the optimization of the hydrophobicity cut-off are followed in Table 2 by an '*'.

Name = Identification of the protein in SwissProt or NRL3d code
len = length of the sequence
OTM = number of observed transmembrane segments
PTM = number of predicted transmembrane segments
Q = Agreement factor
C = Correlation coefficient
SM = Ratio of segment matches
Observed segments = boundaries of observed segments
Predicted segments = boundaries of predicted segments

Name len OTM PTM Q C SM Predicted segments Observed segments

1PRCH 258 1 1 91.69 0.85 1.000 15-32 12-30

1PRCL 273 5 5 86.88 0.76 1.000 30-49, 83-102, 111-131, 177-197, 232-250 29-53, 84-102, 116-139, 171-198, 226-249

1PRCM 323 5 5 86.26 0.76 1.000 49-71, 110-129, 144-161, 204-225, 266-284 52-76, 111-137, 143-166, 198-223, 260-284

4F2_HUMAN 529 1 1 100.00 1.00 1.000 82-104 82-104

5H2A_CRIGR 471 7 7 89.91 0.83 1.000 79-99, 121-139, 150-171, 196-215, 234-252, 324-341, 364-383 76-99, 111-132, 148-171, 192-215, 234-254, 325-346, 363-384

5HT3_HUMAN 478 4 4 93.61 0.90 1.000 243-264, 274-291, 304-321, 452-472 242-268, 274-292, 303-321, 456-475

A15_HUMAN 244 4 4 91.48 0.85 1.000 13-31, 56-74, 85-107, 210-229 12-35, 52-70, 82-107, 209-229

A2AA_HUMAN 450 7 7 90.39 0.84 1.000 39-59, 70-89, 108-129, 153-172, 197-217, 374-392, 410-429 34-59, 71-96, 107-129, 150-173, 193-217, 375-399, 407-430

A4_HUMAN 770 1 1 95.83 0.96 1.000 702-723 700-723

AA1R_CANFA 326 7 7 90.24 0.81 1.000 17-34, 47-69, 81-103, 124-144, 185-207, 236-253, 268-290 11-33, 47-69, 81-102, 124-146, 177-201, 236-259, 268-292

ADT_RICPR 498 12 12 86.19 0.73 1.000 28-45, 61-80, 93-112, 148-168, 186-206, 219-235, 279-297, 317-337, 351-370, 378-396, 443-461, 467-482 34-54, 68-88, 93-113, 148-168, 185-205, 219-239, 280-300, 321-341, 349-369, 380-400, 439-459, 466-486

ALKB_PSEOL 401 6 6 83.18 0.72 0.913 20-39, 44-62, 89-109, 116-134, 228-246, 329-348 22-67, 89-111, 114-134, 146-166, 231-263, 329-349

ATPL_ECOLI 79 2 2 91.18 0.82 1.000 8-28, 53-72 11-31, 53-73

BACH_HALSS 284 7 6 78.76 0.58 0.926 33-53, 66-86, 115-133, 139-158, 167-185, 206-223 33-56, 68-91, 109-127, 138-161, 165-189, 198-221, 233-256

BACR_HALHA 262 7 6 88.51 0.77 0.926 23-42, 55-75, 121-140, 148-169, 190-212, 219-237 23-42, 57-76, 95-114, 121-140, 148-167, 191-210, 217-236

C561_BOVIN 273 6 6 90.93 0.83 1.000 39-59, 77-94, 111-128, 148-167, 183-201, 221-241 38-60, 75-97, 107-129, 145-167, 185-207, 219-241

CB21_PEA 228 3 2 82.76 0.77 0.816 114-134, 182-198 62-81, 114-134, 182-198

CD37_HUMAN 281 4 4 90.68 0.83 1.000 20-38, 60-80, 86-108, 245-266 13-36, 60-74, 86-111, 242-266

CD81_HUMAN 236 4 4 92.71 0.88 1.000 13-35, 58-78, 94-114, 202-218 12-35, 58-78, 90-115, 202-226

CEK2_CHICK 806 1 2 88.85 0.64 0.707 6-23, 369-388 365-389

COX2_PARDE 298 2 3 80.32 0.61 0.816 10-29, 66-87, 108-130 56-88, 103-134

COX3_PARDE 273 7 7 82.52 0.56 1.000 18-35, 49-67, 90-109, 146-164, 172-191, 214-232, 249-267 15-35, 48-76, 79-114, 139-165, 168-196, 203-236, 244-273

CX1B_PARDE 558 12 12 82.08 0.63 1.000 31-49, 95-112, 133-151, 181-201, 224-242, 270-290, 302-323, 338-359, 373-393, 414-432, 449-467, 491-508 29-59, 84-121, 130-151, 178-206, 218-251, 263-298, 304-322, 334-362, 370-395, 404-430, 441-468, 483-513

CXB1_RAT 283 4 4 89.25 0.79 1.000 23-40, 76-96, 136-156, 192-214 20-40, 76-96, 143-163, 189-209

CYDA_ECOLI 522 7 9 85.88 0.67 0.882 20-36, 54-73, 100-118, 127-146, 188-208, 220-238, 392-412, 425-444, 475-494 23-42, 95-114, 130-149, 188-207, 220-239, 393-412, 471-490

CYDB_ECOLI 379 8 8 90.87 0.82 1.000 9-24, 80-99, 121-138, 161-181, 206-226, 264-281, 290-311, 342-360 9-28, 80-99, 123-142, 165-184, 206-225, 263-282, 293-312, 337-356

CYOA_ECOLI 315 2 3 88.55 0.64 0.816 10-30, 48-67, 90-108 51-69, 93-111

CYOC_ECOLI 204 5 5 76.49 0.53 1.000 26-45, 74-91, 98-115, 142-162, 177-198 32-50, 67-85, 102-120, 143-161, 185-203

CYOD_ECOLI 109 3 3 94.32 0.89 1.000 19-37, 45-66, 81-100 18-36, 46-64, 81-99

CYOE_ECOLI 296 7 9 66.64 0.34 0.882 12-29, 38-56, 87-105, 108-128, 133-154, 164-183, 209-229, 233-249, 264-281 10-28, 38-56, 79-97, 108-126, 198-216, 229-247, 269-287

DHG_ECOLI 796 5 6 86.41 0.68 0.913 11-29, 37-58, 63-81, 87-106, 113-130, 707-727 11-37, 41-58, 63-81, 96-110, 119-141

DMSC_ECOLI 287 8 8 91.68 0.81 1.000 10-32, 44-61, 88-107, 117-134, 155-176, 183-201, 223-242, 252-270 10-32, 44-66, 88-107, 113-134, 153-176, 183-203, 223-243, 255-281

DSBB_ECOLI 176 4 3 80.83 0.64 0.866 14-32, 42-63, 145-162 15-31, 50-65, 72-89, 145-162

EDG1_HUMAN 381 7 5 80.67 0.69 0.845 50-71, 122-140, 160-177, 202-221, 257-277 47-71, 79-107, 122-140, 160-185, 202-222, 256-277, 294-314

EGFR_HUMAN 1210 1 1 91.30 0.91 1.000 650-668 646-668

ENVZ_ECOLI 450 2 2 95.12 0.95 1.000 16-35, 163-179 16-35, 162-182

EXBB_ECOLI 244 3 3 88.00 0.76 1.000 25-42, 142-160, 177-195 25-42, 132-150, 178-195

EXBD_ECOLI 141 1 1 100.00 1.00 1.000 26-43 26-43

FCE2_HUMAN 321 1 1 92.31 0.91 1.000 24-45 22-47

FTSH_ECOLI 644 2 2 90.00 0.89 1.000 6-22, 102-120 5-24, 96-120

FTSL_ECOLI 121 1 1 100.00 1.00 1.000 38-57 38-57

FUCP_ECOLI 438 12 11 88.26 0.76 0.957 24-41, 65-85, 92-109, 117-134, 164-181, 212-228, 289-310, 327-345, 352-373, 386-404, 407-428 24-44, 65-85, 92-112, 114-134, 161-181, 214-234, 262-282, 291-311, 327-347, 349-369, 385-405, 408-428

GAA5_HUMAN 462 4 4 94.38 0.93 1.000 260-281, 290-307, 322-341, 431-449 260-281, 286-307, 319-341, 428-449

GLPC_HUMAN 128 1 1 97.92 0.97 1.000 59-81 58-81

GLP_PIG 133 1 1 93.48 0.92 1.000 66-85 63-85

GMCR_HUMAN 400 1 1 88.46 0.87 1.000 327-346 321-346

GPT_CRILO 408 10 8 83.38 0.68 0.949 11-32, 59-76, 94-114, 126-145, 157-179, 222-240, 262-280, 379-397 7-32, 58-79, 95-114, 126-145, 165-184, 195-211, 222-240, 253-269, 275-294, 379-397

GRB_RAT 496 4 2 74.12 0.66 0.707 266-286, 331-350 266-290, 299-316, 331-354, 478-495

HEMA_CDVO 604 1 1 92.86 0.92 1.000 38-55 35-55

HEMA_PI4HA 573 1 1 97.50 0.97 1.000 28-46 28-47

HG2A_HUMAN 296 1 1 88.46 0.87 1.000 53-72 47-72

HISM_SALTY 235 5 3 77.71 0.63 0.775 27-46, 59-79, 203-221 27-47, 59-79, 105-125, 158-178, 200-220

HOXN_ALCEU 351 8 6 69.51 0.41 0.866 20-42, 89-111, 128-149, 230-247, 278-297, 320-338 20-40, 52-72, 95-115, 129-149, 200-220, 244-264, 270-290, 317-337

IG1R_HUMAN 1367 1 1 97.92 0.98 1.000 936-958 936-959

IL2A_HUMAN 272 1 1 100.00 1.00 1.000 241-259 241-259

IL2B_HUMAN 551 1 2 86.19 0.59 0.707 12-30, 247-265 241-265

IM23_SCHMA 218 4 4 92.80 0.88 1.000 14-32, 56-72, 88-107, 184-204 13-36, 56-71, 83-108, 184-205

IMM1_ECOLI 113 3 2 78.02 0.59 0.816 6-24, 86-104 9-25, 39-57, 84-104

IMMA_CITFR 178 4 3 82.40 0.69 0.866 18-35, 106-123, 145-165 14-37, 69-89, 107-123, 143-165

ITA5_MOUSE 1053 1 1 86.54 0.85 1.000 1003-1021 1000-1025

KDPD_ECOLI 894 3 4 97.62 0.97 1.000 403-423, 425-442, 446-465, 478-498 403-444, 446-466, 478-498

KGTP_ECOLI 432 12 11 87.42 0.73 0.957 35-53, 66-84, 96-113, 166-183, 195-214, 244-262, 274-295, 312-330, 340-360, 371-389, 405-423 26-54, 62-80, 96-116, 120-136, 163-185, 196-214, 244-261, 275-301, 312-330, 337-360, 369-392, 403-423

LACY_ECOLI 417 12 11 85.97 0.70 0.957 9-27, 47-66, 76-95, 103-125, 145-164, 167-187, 222-239, 260-283, 291-309, 349-369, 383-400 11-33, 47-67, 75-99, 103-125, 145-163, 168-187, 212-234, 260-281, 291-310, 315-334, 347-366, 380-399

LECI_MOUSE 301 1 1 95.24 0.95 1.000 59-77 59-79

LEP_ECOLI 324 2 2 91.41 0.85 1.000 6-22, 63-81 4-22, 59-77

LSPA_ECOLI 164 4 4 92.57 0.83 1.000 12-31, 69-88, 96-113, 134-153 12-22, 70-88, 96-113, 139-153

MAGL_MOUSE 637 1 1 92.50 0.92 1.000 517-533 517-536

MALF_ECOLI 514 8 8 95.82 0.89 1.000 19-36, 40-58, 72-91, 277-294, 319-336, 371-392, 429-447, 484-504 17-35, 40-58, 73-91, 277-295, 319-337, 371-389, 428-436, 486-504

MALG_ECOLI 296 6 6 85.28 0.71 1.000 18-37, 91-111, 124-144, 160-177, 205-227, 263-281 19-39, 82-102, 124-144, 151-171, 205-225, 260-280

MELB_ECOLI 469 12 11 87.31 0.75 1.000 16-37, 75-93, 102-119, 146-163, 177-194, 232-251, 263-282, 293-314, 320-340, 377-393, 408-426 8-28, 33-53, 76-96, 103-123, 146-166, 172-192, 231-251, 263-283, 293-313, 320-340, 370-390, 408-428

MOTA_ECOLI 295 4 3 76.51 0.57 0.866 8-28, 165-185, 198-220 2-21, 34-51, 171-191, 201-222

MOTB_ECOLI 308 1 1 88.64 0.87 1.000 33-49 28-49

MPRD_HUMAN 277 1 1 88.00 0.86 1.000 191-209 186-210

MTR_ECOLI 414 11 9 78.42 0.56 0.905 13-32, 39-56, 95-114, 129-146, 152-172, 228-246, 284-306, 344-362, 387-407 16-36, 42-62, 89-109, 129-149, 152-172, 191-211, 229-249, 256-276, 286-306, 326-367, 387-407

MYP0_HUMAN 248 1 2 85.65 0.58 0.707 13-33, 156-176 154-179

NEP_HUMAN 749 1 1 89.13 0.88 1.000 32-49 28-50

NGFR_HUMAN 427 1 1 93.18 0.93 1.000 254-272 251-272

OPPB_SALTY 306 6 6 92.17 0.85 1.000 16-37, 103-121, 138-156, 173-190, 229-250, 274-293 10-30, 100-121, 138-158, 173-190, 227-250, 272-293

OPPC_SALTY 302 6 5 88.27 0.80 1.000 38-56, 103-121, 149-171, 218-236, 270-289 38-59, 103-122, 140-160, 164-180, 216-236, 268-290

OPS1_CALVI 371 7 5 79.19 0.64 0.845 50-73, 123-144, 168-186, 218-239, 275-293 48-72, 85-110, 125-144, 164-187, 212-239, 275-298, 306-330

OPSB_HUMAN 348 7 6 84.01 0.72 0.926 38-58, 76-94, 112-130, 153-172, 202-218, 250-270 34-58, 71-96, 111-130, 150-173, 200-227, 250-273, 282-306

PHOR_ECOLI 431 2 1 72.10 0.58 1.000 22-44 14-34, 39-59

PIGR_HUMAN 764 1 1 97.83 0.98 1.000 640-661 639-661

RFBP_SALTY 476 5 5 94.35 0.87 1.000 22-40, 56-72, 90-107, 115-131, 285-303 15-31, 56-72, 90-106, 115-131, 285-301

RHAT_ECOLI 344 10 10 91.67 0.82 1.000 7-26, 40-58, 74-94, 103-121, 136-152, 174-192, 217-234, 259-278, 290-310, 320-338 4-24, 38-58, 74-94, 101-121, 137-157, 175-195, 214-234, 259-279, 290-310, 323-343

RIB1_RAT 605 1 1 100.00 1.00 1.000 438-455 438-455

SECD_ECOLI 615 6 5 83.81 0.76 1.000 10-26, 456-472, 475-494, 499-518, 577-596 10-30, 452-472, 476-497, 504-524, 564-584, 586-605

SECE_ECOLI 127 3 3 98.21 0.97 1.000 19-36, 45-62, 94-111 19-36, 45-63, 93-111

SECY_ECOLI 443 10 10 91.33 0.83 1.000 23-41, 77-98, 122-140, 154-174, 189-207, 217-237, 274-294, 318-337, 369-387, 397-414 23-42, 75-95, 122-139, 154-174, 183-203, 217-237, 274-294, 316-335, 376-395, 399-414

SSRG_RAT 185 4 3 83.80 0.72 0.866 30-48, 59-77, 136-157 28-48, 55-76, 136-157, 164-184

SUIS_HUMAN 1827 1 2 97.00 0.69 0.707 13-31, 622-639 13-32

TAL6_HUMAN 202 4 4 93.16 0.88 1.000 10-30, 46-68, 94-113, 162-179 10-30, 46-70, 89-116, 162-182

TCB1_RABIT 319 1 1 93.18 0.92 1.000 293-311 292-313

TCR1_ECOLI 399 11 12 92.28 0.84 1.000 7-28, 47-66, 74-93, 104-121, 133-154, 163-181, 215-236, 248-268, 279-297, 300-320, 340-361, 368-385 8-28, 44-64, 76-96, 104-124, 133-153, 161-181, 216-236, 246-267, 279-320, 338-358, 365-385

TOLR_ECOLI 142 1 1 84.00 0.80 1.000 24-40 16-40

TRBM_HUMAN 575 1 1 91.67 0.91 1.000 517-536 516-539

TRSR_HUMAN 760 1 2 81.40 0.54 0.707 71-87, 540-558 63-88

UHPT_ECOLI 463 12 11 90.81 0.82 0.957 27-45, 56-76, 97-116, 119-136, 160-181, 191-210, 260-278, 300-318, 327-344, 357-379, 426-446 28-45, 56-80, 97-113, 120-136, 159-183, 191-210, 260-278, 301-318, 327-344, 352-376, 388-406, 422-446

UPKB_BOVIN 259 4 4 95.21 0.92 1.000 12-30, 59-80, 86-106, 229-248 12-37, 60-80, 86-106, 229-249

VMT2_IAANN 97 1 1 96.59 0.93 1.000 26-43 25-42

VNB_INBBE 99 1 1 95.45 0.94 1.000 21-40 19-40

Average 88.83 0.80 0.954

Table 2: Set of 155 proteins used as a test set for the PRED-TMR algorithm.

Name len OTM PTM Q C Predicted segments Observed segments

1PRCH* 258 1 1 91.69 0.85 15-32 12-30

1PRCL* 273 5 5 86.88 0.76 30-49, 83-102, 111-131, 177-197, 232-250 29-53, 84-102, 116-139, 171-198, 226-249

1PRCM* 323 5 5 86.26 0.76 49-71, 110-129, 144-161, 204-225, 266-284 52-76, 111-137, 143-166, 198-223, 260-284

4F2_HUMAN* 529 1 1 100.00 1.00 82-104 82-104

5H1A_HUMAN 422 7 6 86.01 0.77 41-63, 74-96, 111-132, 153-173, 197-215, 349-368 37-62, 74-98, 110-132, 153-178, 192-217, 346-367, 379-403

5H2A_CRIGR 471 7 7 89.91 0.83 79-99, 121-139, 150-171, 196-215, 234-252, 324-341, 364-383 76-99, 111-132, 148-171, 192-215, 234-254, 325-346, 363-384

5HT3_HUMAN 478 4 4 93.61 0.90 243-264, 274-291, 304-321, 452-472 242-268, 274-292, 303-321, 456-475

5HT3_MOUSE* 487 4 4 90.31 0.84 247-266, 282-303, 307-324, 465-481 246-272, 278-296, 306-324, 465-484

A15_HUMAN 244 4 4 91.48 0.85 13-31, 56-74, 85-107, 210-229 12-35, 52-70, 82-107, 209-229

A1AA_HUMAN* 466 7 6 82.03 0.70 26-43, 73-94, 101-122, 143-163, 185-203, 274-292 28-51, 65-88, 100-122, 144-167, 182-205, 274-297, 306-329

A2AA_HUMAN* 450 7 7 90.39 0.84 39-59, 70-89, 108-129, 153-172, 197-217, 374-392, 410-429 34-59, 71-96, 107-129, 150-173, 193-217, 375-399, 407-430

A4_HUMAN* 770 1 1 95.83 0.96 702-723 700-723

AA1R_CANFA* 326 7 7 90.24 0.81 17-34, 47-69, 81-103, 124-144, 185-207, 236-253, 268-290 11-33, 47-69, 81-102, 124-146, 177-201, 236-259, 268-292

AA2A_CANFA* 412 7 6 80.09 0.64 14-33, 53-73, 78-100, 123-143, 182-204, 235-252 8-30, 44-66, 78-100, 121-143, 174-198, 235-258, 267-290

ADT_RICPR* 498 12 12 86.19 0.73 28-45, 61-80, 93-112, 148-168, 186-206, 219-235, 279-297, 317-337, 351-370, 378-396, 443-461, 467-482 34-54, 68-88, 93-113, 148-168, 185-205, 219-239, 280-300, 321-341, 349-369, 380-400, 439-459, 466-486

ALKB_PSEOL 401 6 6 83.18 0.72 20-39, 44-62, 89-109, 116-134, 228-246, 329-348 22-67, 89-111, 114-134, 146-166, 231-263, 329-349

ATPL_ECOLI 79 2 2 91.18 0.82 8-28, 53-72 11-31, 53-73

BAC1_HALS1 260 7 6 86.83 0.73 23-41, 57-78, 120-139, 148-165, 186-203, 218-236 23-44, 56-81, 96-113, 120-139, 150-170, 186-205, 213-236

BACH_HALHM* 206 7 5 73.50 0.44 25-45, 80-98, 104-123, 132-150, 171-188 1-15, 27-50, 74-92, 103-126, 130-154, 163-186, 198-206

BACH_HALSS 284 7 6 78.76 0.58 33-53, 66-86, 115-133, 139-158, 167-185, 206-223 33-56, 68-91, 109-127, 138-161, 165-189, 198-221, 233-256

BACR_HALHA* 262 7 6 88.51 0.77 23-42, 55-75, 121-140, 148-169, 190-212, 219-237 23-42, 57-76, 95-114, 121-140, 148-167, 191-210, 217-236

C561_BOVIN 273 6 6 90.93 0.83 39-59, 77-94, 111-128, 148-167, 183-201, 221-241 38-60, 75-97, 107-129, 145-167, 185-207, 219-241

CB21_PEA* 228 3 2 82.76 0.77 114-134, 182-198 62-81, 114-134, 182-198

CD37_HUMAN 281 4 4 90.68 0.83 20-38, 60-80, 86-108, 245-266 13-36, 60-74, 86-111, 242-266

CD53_HUMAN 219 4 4 90.12 0.82 18-36, 55-72, 85-105, 182-202 11-36, 55-69, 81-106, 182-206

CD63_HUMAN 237 4 4 92.65 0.88 11-30, 54-70, 85-106, 202-222 11-34, 51-69, 81-106, 203-223

CD63_RAT 237 4 4 92.65 0.88 11-30, 54-70, 85-106, 202-222 11-34, 51-69, 81-106, 203-223

CD81_HUMAN 236 4 4 92.71 0.88 13-35, 58-78, 94-114, 202-218 12-35, 58-78, 90-115, 202-226

CD82_HUMAN 267 4 4 88.19 0.78 15-36, 58-78, 89-108, 228-248 11-34, 54-72, 84-110, 229-250

CD9_CERAE 227 4 4 83.75 0.72 18-34, 59-77, 86-101, 194-212 11-34, 55-75, 87-110, 195-220

CD9_FELCA 225 4 4 83.72 0.72 18-34, 57-75, 84-99, 192-210 11-34, 53-73, 85-108, 193-218

CD9_HUMAN 227 4 4 83.75 0.72 18-34, 59-77, 86-101, 194-212 11-34, 55-75, 87-110, 195-220

CEK2_CHICK* 806 1 2 88.85 0.64 6-23, 369-388 365-389

CO02_HUMAN 237 4 4 85.40 0.74 17-33, 58-78, 83-98, 206-226 10-33, 58-72, 84-109, 206-230

COX2_PARDE 298 2 3 80.32 0.61 10-29, 66-87, 108-130 56-88, 103-134

COX3_PARDE 273 7 7 82.52 0.56 18-35, 49-67, 90-109, 146-164, 172-191, 214-232, 249-267 15-35, 48-76, 79-114, 139-165, 168-196, 203-236, 244-273

CX1B_PARDE 558 12 12 82.08 0.63 31-49, 95-112, 133-151, 181-201, 224-242, 270-290, 302-323, 338-359, 373-393, 414-432, 449-467, 491-508 29-59, 84-121, 130-151, 178-206, 218-251, 263-298, 304-322, 334-362, 370-395, 404-430, 441-468, 483-513

CXB1_HUMAN 283 4 4 89.25 0.79 23-40, 76-96, 136-156, 192-214 20-40, 76-96, 143-163, 189-209

CXB1_RAT 283 4 4 89.25 0.79 23-40, 76-96, 136-156, 192-214 20-40, 76-96, 143-163, 189-209

CXB1_XENLA 264 4 4 88.93 0.78 23-40, 76-96, 136-156, 192-214 20-40, 76-96, 143-163, 189-209

CYDA_ECOLI 522 7 9 85.88 0.67 20-36, 54-73, 100-118, 127-146, 188-208, 220-238, 392-412, 425-444, 475-494 23-42, 95-114, 130-149, 188-207, 220-239, 393-412, 471-490

CYDB_ECOLI 379 8 8 90.87 0.82 9-24, 80-99, 121-138, 161-181, 206-226, 264-281, 290-311, 342-360 9-28, 80-99, 123-142, 165-184, 206-225, 263-282, 293-312, 337-356

CYOA_ECOLI* 315 2 3 88.55 0.64 10-30, 48-67, 90-108 51-69, 93-111

CYOB_ECOLI* 663 15 15 79.34 0.59 16-35, 59-79, 108-127, 136-156, 194-213, 237-255, 287-305, 310-332, 335-357, 385-403, 423-440, 457-477, 494-514, 591-608, 610-627 17-35, 58-76, 102-121, 144-162, 195-213, 232-250, 277-296, 320-339, 348-366, 382-401, 410-429, 457-476, 494-513, 588-607, 614-634

CYOC_ECOLI* 204 5 5 76.49 0.53 26-45, 74-91, 98-115, 142-162, 177-198 32-50, 67-85, 102-120, 143-161, 185-203

CYOD_ECOLI* 109 3 3 94.32 0.89 19-37, 45-66, 81-100 18-36, 46-64, 81-99

CYOE_ECOLI* 296 7 9 66.64 0.34 12-29, 38-56, 87-105, 108-128, 133-154, 164-183, 209-229, 233-249, 264-281 10-28, 38-56, 79-97, 108-126, 198-216, 229-247, 269-287

DHG_ECOLI 796 5 6 86.41 0.68 11-29, 37-58, 63-81, 87-106, 113-130, 707-727 11-37, 41-58, 63-81, 96-110, 119-141

DMSC_ECOLI 287 8 8 91.68 0.81 10-32, 44-61, 88-107, 117-134, 155-176, 183-201, 223-242, 252-270 10-32, 44-66, 88-107, 113-134, 153-176, 183-203, 223-243, 255-281

DSBB_ECOLI 176 4 3 80.83 0.64 14-32, 42-63, 145-162 15-31, 50-65, 72-89, 145-162

EDG1_HUMAN* 381 7 5 80.67 0.69 50-71, 122-140, 160-177, 202-221, 257-277 47-71, 79-107, 122-140, 160-185, 202-222, 256-277, 294-314

EGFR_CHICK 703 1 2 97.97 0.55 14-31, 655-677 655-667

EGFR_HUMAN* 1210 1 1 91.30 0.91 650-668 646-668

ENVZ_ECOLI 450 2 2 95.12 0.95 16-35, 163-179 16-35, 162-182

EXBB_ECOLI 244 3 3 88.00 0.76 25-42, 142-160, 177-195 25-42, 132-150, 178-195

EXBD_ECOLI 141 1 1 100.00 1.00 26-43 26-43

FCE2_HUMAN* 321 1 1 92.31 0.91 24-45 22-47

FTSH_ECOLI 644 2 2 90.00 0.89 6-22, 102-120 5-24, 96-120

FTSL_ECOLI 121 1 1 100.00 1.00 38-57 38-57

FUCP_ECOLI 438 12 11 88.26 0.76 24-41, 65-85, 92-109, 117-134, 164-181, 212-228, 289-310, 327-345, 352-373, 386-404, 407-428 24-44, 65-85, 92-112, 114-134, 161-181, 214-234, 262-282, 291-311, 327-347, 349-369, 385-405, 408-428

GAA1_CHICK 455 4 4 93.98 0.91 253-274, 283-300, 315-334, 424-442 252-273, 279-300, 313-334, 421-441

GAA1_HUMAN 456 4 4 94.18 0.92 253-274, 283-300, 315-334, 425-443 252-273, 279-300, 313-334, 422-443

GAA2_HUMAN 451 4 4 89.65 0.86 253-274, 283-300, 315-334, 423-441 252-273, 279-300, 313-334, 420-451

GAA3_HUMAN 492 4 4 94.19 0.92 278-299, 308-325, 340-359, 461-479 277-298, 304-325, 338-359, 458-479

GAA4_BOVIN 556 4 5 93.16 0.82 10-30, 259-280, 289-306, 322-341, 526-544 259-280, 285-306, 319-341, 526-545

GAA5_HUMAN 462 4 4 94.38 0.93 260-281, 290-307, 322-341, 431-449 260-281, 286-307, 319-341, 428-449

GAA6_MOUSE 443 4 4 93.89 0.92 233-254, 263-280, 295-314, 413-431 233-254, 259-280, 291-314, 410-431

GAB1_HUMAN 474 4 2 66.61 0.46 243-263, 314-333 246-267, 271-293, 305-327, 452-473

GAB2_HUMAN 474 4 3 64.39 0.34 8-24, 242-262, 313-332 245-266, 270-292, 304-326, 452-473

GAB3_HUMAN 473 4 3 64.38 0.34 9-25, 243-263, 314-333 246-267, 271-293, 305-327, 451-472

GAB4_CHICK 488 4 2 66.85 0.47 242-262, 313-332 245-266, 271-292, 304-326, 466-487

GAB_LYMST 499 4 2 70.51 0.58 249-269, 314-333 252-273, 278-299, 311-333, 476-499

GAC1_RAT 465 4 2 69.66 0.54 270-290, 329-349 273-294, 299-320, 332-354, 445-465

GAC3_MOUSE 467 4 2 69.22 0.54 253-273, 312-332 255-277, 281-303, 315-337, 444-467

GAC4_CHICK 457 4 2 71.09 0.58 258-279, 314-334 257-279, 283-305, 317-339, 434-457

GAD_MOUSE 449 4 3 80.29 0.73 248-267, 280-297, 312-330 249-271, 275-297, 309-331, 427-449

GAR1_HUMAN 473 4 2 71.61 0.61 275-295, 340-359 276-299, 303-325, 337-359, 452-473

GAR2_HUMAN 465 4 3 79.76 0.70 261-281, 296-315, 326-345 262-285, 289-311, 323-345, 444-465

GLPA_HUMAN* 150 1 2 82.64 0.53 6-27, 92-110 92-114

GLPC_HUMAN* 128 1 1 97.92 0.97 59-81 58-81

GLPT_ECOLI 452 12 11 90.22 0.80 28-44, 65-86, 94-113, 115-133, 166-183, 188-208, 254-273, 322-341, 351-370, 384-404, 417-436 28-44, 65-87, 98-113, 120-138, 167-183, 186-208, 254-273, 293-310, 322-341, 351-373, 382-406, 415-437

GLP_PIG* 133 1 1 93.48 0.92 66-85 63-85

GMCR_HUMAN* 400 1 1 88.46 0.87 327-346 321-346

GPT_CRILO* 408 10 8 83.38 0.68 11-32, 59-76, 94-114, 126-145, 157-179, 222-240, 262-280, 379-397 7-32, 58-79, 95-114, 126-145, 165-184, 195-211, 222-240, 253-269, 275-294, 379-397

GRA1_HUMAN 449 4 3 82.18 0.77 255-272, 313-332, 421-438 248-274, 281-298, 313-336, 421-438

GRA2_HUMAN 452 4 3 82.18 0.77 261-278, 319-338, 424-441 254-280, 287-304, 319-342, 424-441

GRA3_RAT 464 4 4 78.57 0.61 10-28, 260-277, 318-337, 434-451 253-279, 286-303, 315-341, 434-451

GRB_RAT 496 4 2 74.12 0.66 266-286, 331-350 266-290, 299-316, 331-354, 478-495

HEMA_CDVO* 604 1 1 92.86 0.92 38-55 35-55

HEMA_MEASI* 617 1 1 95.83 0.96 37-58 35-58

HEMA_PI4HA* 573 1 1 97.50 0.97 28-46 28-47

HG2A_HUMAN* 296 1 1 88.46 0.87 53-72 47-72

HISM_SALTY 235 5 3 77.71 0.63 27-46, 59-79, 203-221 27-47, 59-79, 105-125, 158-178, 200-220

HISQ_SALTY 228 5 4 78.86 0.60 19-37, 62-80, 93-112, 193-215 13-33, 59-79, 88-108, 153-173, 195-215

HOXN_ALCEU 351 8 6 69.51 0.41 20-42, 89-111, 128-149, 230-247, 278-297, 320-338 20-40, 52-72, 95-115, 129-149, 200-220, 244-264, 270-290, 317-337

IG1R_HUMAN 1367 1 1 97.92 0.98 936-958 936-959

IL2A_HUMAN* 272 1 1 100.00 1.00 241-259 241-259

IL2B_HUMAN* 551 1 2 86.19 0.59 12-30, 247-265 241-265

IM23_SCHJA 218 4 4 88.80 0.80 14-32, 56-73, 81-97, 184-204 13-36, 56-71, 83-108, 184-205

IM23_SCHMA 218 4 4 92.80 0.88 14-32, 56-72, 88-107, 184-204 13-36, 56-71, 83-108, 184-205

IMM1_ECOLI 113 3 2 78.02 0.59 6-24, 86-104 9-25, 39-57, 84-104

IMMA_CITFR 178 4 3 82.40 0.69 18-35, 106-123, 145-165 14-37, 69-89, 107-123, 143-165

ITA5_MOUSE* 1053 1 1 86.54 0.85 1003-1021 1000-1025

KDPD_ECOLI 894 3 4 97.62 0.97 403-423, 425-442, 446-465, 478-498 403-444, 446-466, 478-498

KGTP_ECOLI 432 12 11 87.42 0.73 35-53, 66-84, 96-113, 166-183, 195-214, 244-262, 274-295, 312-330, 340-360, 371-389, 405-423 26-54, 62-80, 96-116, 120-136, 163-185, 196-214, 244-261, 275-301, 312-330, 337-360, 369-392, 403-423

LACY_ECOLI* 417 12 11 85.97 0.70 9-27, 47-66, 76-95, 103-125, 145-164, 167-187, 222-239, 260-283, 291-309, 349-369, 383-400 11-33, 47-67, 75-99, 103-125, 145-163, 168-187, 212-234, 260-281, 291-310, 315-334, 347-366, 380-399

LECH_HUMAN* 290 1 1 95.24 0.95 40-58 40-60

LECI_MOUSE* 301 1 1 95.24 0.95 59-77 59-79

LEP_ECOLI* 324 2 2 91.41 0.85 6-22, 63-81 4-22, 59-77

LHA4_RHOAC 53 1 1 64.51 0.29 6-26 15-35

LHB5_RHOAC 41 1 0 50.00 0.00 14-36

LSPA_ECOLI 164 4 4 92.57 0.83 12-31, 69-88, 96-113, 134-153 12-22, 70-88, 96-113, 139-153

MAGL_MOUSE* 637 1 1 92.50 0.92 517-533 517-536

MALF_ECOLI* 514 8 8 95.82 0.89 19-36, 40-58, 72-91, 277-294, 319-336, 371-392, 429-447, 484-504 17-35, 40-58, 73-91, 277-295, 319-337, 371-389, 428-436, 486-504

MALG_ECOLI 296 6 6 85.28 0.71 18-37, 91-111, 124-144, 160-177, 205-227, 263-281 19-39, 82-102, 124-144, 151-171, 205-225, 260-280

MELB_ECOLI 469 12 11 87.31 0.75 16-37, 75-93, 102-119, 146-163, 177-194, 232-251, 263-282, 293-314, 320-340, 377-393, 408-426 8-28, 33-53, 76-96, 103-123, 146-166, 172-192, 231-251, 263-283, 293-313, 320-340, 370-390, 408-428

MOTA_ECOLI 295 4 3 76.51 0.57 8-28, 165-185, 198-220 2-21, 34-51, 171-191, 201-222

MOTB_ECOLI* 308 1 1 88.64 0.87 33-49 28-49

MPRD_HUMAN* 277 1 1 88.00 0.86 191-209 186-210

MTR_ECOLI 414 11 9 78.42 0.56 13-32, 39-56, 95-114, 129-146, 152-172, 228-246, 284-306, 344-362, 387-407 16-36, 42-62, 89-109, 129-149, 152-172, 191-211, 229-249, 256-276, 286-306, 326-367, 387-407

MYP0_HUMAN* 248 1 2 85.65 0.58 13-33, 156-176 154-179

NEP_HUMAN* 749 1 1 89.13 0.88 32-49 28-50

NGFR_HUMAN* 427 1 1 93.18 0.93 254-272 251-272

OPPB_SALTY* 306 6 6 92.17 0.85 16-37, 103-121, 138-156, 173-190, 229-250, 274-293 10-30, 100-121, 138-158, 173-190, 227-250, 272-293

OPPC_SALTY* 302 6 5 88.27 0.80 38-56, 103-121, 149-171, 218-236, 270-289 38-59, 103-122, 140-160, 164-180, 216-236, 268-290

OPS1_CALVI* 371 7 5 79.19 0.64 50-73, 123-144, 168-186, 218-239, 275-293 48-72, 85-110, 125-144, 164-187, 212-239, 275-298, 306-330

OPS2_DROME* 381 7 5 78.98 0.64 59-82, 132-153, 177-195, 227-248, 289-308 57-81, 94-119, 134-153, 173-196, 221-248, 284-307, 315-339

OPS3_DROME* 383 7 5 77.07 0.61 64-83, 132-153, 172-191, 219-239, 289-309 58-82, 95-119, 134-153, 172-196, 221-248, 285-308, 317-341

OPS4_DROME* 378 7 5 77.33 0.61 59-79, 128-149, 173-191, 216-234, 285-305 54-78, 91-113, 130-149, 168-192, 217-244, 281-304, 313-337

OPSB_HUMAN* 348 7 6 84.01 0.72 38-58, 76-94, 112-130, 153-172, 202-218, 250-270 34-58, 71-96, 111-130, 150-173, 200-227, 250-273, 282-306

OPSD_BOVIN* 348 7 6 82.53 0.68 37-54, 84-105, 114-133, 153-175, 203-221, 254-276 37-61, 74-98, 114-133, 153-176, 203-230, 253-276, 285-309

OPSG_HUMAN* 364 7 5 79.10 0.65 58-77, 130-149, 168-185, 225-246, 269-290 53-77, 90-115, 130-149, 169-192, 219-246, 269-292, 301-325

OPSR_HUMAN* 364 7 5 79.94 0.66 58-77, 130-149, 170-191, 219-237, 269-290 53-77, 90-115, 130-149, 169-192, 219-246, 269-292, 301-325

PHOR_ECOLI 431 2 1 72.10 0.58 22-44 14-34, 39-59

PIGR_HUMAN* 764 1 1 97.83 0.98 640-661 639-661

RFBP_SALTY 476 5 5 94.35 0.87 22-40, 56-72, 90-107, 115-131, 285-303 15-31, 56-72, 90-106, 115-131, 285-301

RHAT_ECOLI 344 10 10 91.67 0.82 7-26, 40-58, 74-94, 103-121, 136-152, 174-192, 217-234, 259-278, 290-310, 320-338 4-24, 38-58, 74-94, 101-121, 137-157, 175-195, 214-234, 259-279, 290-310, 323-343

RIB1_RAT 605 1 1 100.00 1.00 438-455 438-455

SECD_ECOLI 615 6 5 83.81 0.76 10-26, 456-472, 475-494, 499-518, 577-596 10-30, 452-472, 476-497, 504-524, 564-584, 586-605

SECE_ECOLI* 127 3 3 98.21 0.97 19-36, 45-62, 94-111 19-36, 45-63, 93-111

SECY_BACSU 431 10 9 86.98 0.75 18-36, 67-84, 116-136, 150-167, 170-188, 217-234, 271-291, 310-329, 369-386 18-39, 59-80, 115-132, 148-167, 174-192, 217-234, 268-291, 310-329, 367-386, 392-410

SECY_ECOLI 443 10 10 91.33 0.83 23-41, 77-98, 122-140, 154-174, 189-207, 217-237, 274-294, 318-337, 369-387, 397-414 23-42, 75-95, 122-139, 154-174, 183-203, 217-237, 274-294, 316-335, 376-395, 399-414

SSRG_RAT 185 4 3 83.80 0.72 30-48, 59-77, 136-157 28-48, 55-76, 136-157, 164-184

SUIS_HUMAN* 1827 1 2 97.00 0.69 13-31, 622-639 13-32

TAL6_HUMAN 202 4 4 93.16 0.88 10-30, 46-68, 94-113, 162-179 10-30, 46-70, 89-116, 162-182

TCB1_RABIT* 319 1 1 93.18 0.92 293-311 292-313

TCR1_ECOLI 399 11 12 92.28 0.84 7-28, 47-66, 74-93, 104-121, 133-154, 163-181, 215-236, 248-268, 279-297, 300-320, 340-361, 368-385 8-28, 44-64, 76-96, 104-124, 133-153, 161-181, 216-236, 246-267, 279-320, 338-358, 365-385

TOLQ_ECOLI 230 3 3 80.41 0.69 19-36, 140-159, 174-192 9-36, 127-159, 162-191

TOLR_ECOLI 142 1 1 84.00 0.80 24-40 16-40

TRBM_HUMAN* 575 1 1 91.67 0.91 517-536 516-539

TRSR_HUMAN* 760 1 2 81.40 0.54 71-87, 540-558 63-88

UHPT_ECOLI 463 12 11 90.81 0.82 27-45, 56-76, 97-116, 119-136, 160-181, 191-210, 260-278, 300-318, 327-344, 357-379, 426-446 28-45, 56-80, 97-113, 120-136, 159-183, 191-210, 260-278, 301-318, 327-344, 352-376, 388-406, 422-446

UPKB_BOVIN 259 4 4 95.21 0.92 12-30, 59-80, 86-106, 229-248 12-37, 60-80, 86-106, 229-249

VMT2_IAANN* 97 1 1 96.59 0.93 26-43 25-42

VNB_INBBE* 99 1 1 95.45 0.94 21-40 19-40

Average 86.39 0.75

Name	len	OTM	PTM	Q	C	SM	Predicted segments	Observed segments
1PRCH	258	1	1	91.69	0.85	1.000	15-32	12-30
1PRCL	273	5	5	86.88	0.76	1.000	30-49, 83-102, 111-131, 177-197, 232-250	29-53, 84-102, 116-139, 171-198, 226-249
1PRCM	323	5	5	86.26	0.76	1.000	49-71, 110-129, 144-161, 204-225, 266-284	52-76, 111-137, 143-166, 198-223, 260-284
4F2_HUMAN	529	1	1	100.00	1.00	1.000	82-104	82-104
5H2A_CRIGR	471	7	7	89.91	0.83	1.000	79-99, 121-139, 150-171, 196-215, 234-252, 324-341, 364-383	76-99, 111-132, 148-171, 192-215, 234-254, 325-346, 363-384
5HT3_HUMAN	478	4	4	93.61	0.90	1.000	243-264, 274-291, 304-321, 452-472	242-268, 274-292, 303-321, 456-475
A15_HUMAN	244	4	4	91.48	0.85	1.000	13-31, 56-74, 85-107, 210-229	12-35, 52-70, 82-107, 209-229
A2AA_HUMAN	450	7	7	90.39	0.84	1.000	39-59, 70-89, 108-129, 153-172, 197-217, 374-392, 410-429	34-59, 71-96, 107-129, 150-173, 193-217, 375-399, 407-430
A4_HUMAN	770	1	1	95.83	0.96	1.000	702-723	700-723
AA1R_CANFA	326	7	7	90.24	0.81	1.000	17-34, 47-69, 81-103, 124-144, 185-207, 236-253, 268-290	11-33, 47-69, 81-102, 124-146, 177-201, 236-259, 268-292
ADT_RICPR	498	12	12	86.19	0.73	1.000	28-45, 61-80, 93-112, 148-168, 186-206, 219-235, 279-297, 317-337, 351-370, 378-396, 443-461, 467-482	34-54, 68-88, 93-113, 148-168, 185-205, 219-239, 280-300, 321-341, 349-369, 380-400, 439-459, 466-486
ALKB_PSEOL	401	6	6	83.18	0.72	0.913	20-39, 44-62, 89-109, 116-134, 228-246, 329-348	22-67, 89-111, 114-134, 146-166, 231-263, 329-349
ATPL_ECOLI	79	2	2	91.18	0.82	1.000	8-28, 53-72	11-31, 53-73
BACH_HALSS	284	7	6	78.76	0.58	0.926	33-53, 66-86, 115-133, 139-158, 167-185, 206-223	33-56, 68-91, 109-127, 138-161, 165-189, 198-221, 233-256
BACR_HALHA	262	7	6	88.51	0.77	0.926	23-42, 55-75, 121-140, 148-169, 190-212, 219-237	23-42, 57-76, 95-114, 121-140, 148-167, 191-210, 217-236
C561_BOVIN	273	6	6	90.93	0.83	1.000	39-59, 77-94, 111-128, 148-167, 183-201, 221-241	38-60, 75-97, 107-129, 145-167, 185-207, 219-241
CB21_PEA	228	3	2	82.76	0.77	0.816	114-134, 182-198	62-81, 114-134, 182-198
CD37_HUMAN	281	4	4	90.68	0.83	1.000	20-38, 60-80, 86-108, 245-266	13-36, 60-74, 86-111, 242-266
CD81_HUMAN	236	4	4	92.71	0.88	1.000	13-35, 58-78, 94-114, 202-218	12-35, 58-78, 90-115, 202-226
CEK2_CHICK	806	1	2	88.85	0.64	0.707	6-23, 369-388	365-389
COX2_PARDE	298	2	3	80.32	0.61	0.816	10-29, 66-87, 108-130	56-88, 103-134
COX3_PARDE	273	7	7	82.52	0.56	1.000	18-35, 49-67, 90-109, 146-164, 172-191, 214-232, 249-267	15-35, 48-76, 79-114, 139-165, 168-196, 203-236, 244-273
CX1B_PARDE	558	12	12	82.08	0.63	1.000	31-49, 95-112, 133-151, 181-201, 224-242, 270-290, 302-323, 338-359, 373-393, 414-432, 449-467, 491-508	29-59, 84-121, 130-151, 178-206, 218-251, 263-298, 304-322, 334-362, 370-395, 404-430, 441-468, 483-513
CXB1_RAT	283	4	4	89.25	0.79	1.000	23-40, 76-96, 136-156, 192-214	20-40, 76-96, 143-163, 189-209
CYDA_ECOLI	522	7	9	85.88	0.67	0.882	20-36, 54-73, 100-118, 127-146, 188-208, 220-238, 392-412, 425-444, 475-494	23-42, 95-114, 130-149, 188-207, 220-239, 393-412, 471-490
CYDB_ECOLI	379	8	8	90.87	0.82	1.000	9-24, 80-99, 121-138, 161-181, 206-226, 264-281, 290-311, 342-360	9-28, 80-99, 123-142, 165-184, 206-225, 263-282, 293-312, 337-356
CYOA_ECOLI	315	2	3	88.55	0.64	0.816	10-30, 48-67, 90-108	51-69, 93-111
CYOC_ECOLI	204	5	5	76.49	0.53	1.000	26-45, 74-91, 98-115, 142-162, 177-198	32-50, 67-85, 102-120, 143-161, 185-203
CYOD_ECOLI	109	3	3	94.32	0.89	1.000	19-37, 45-66, 81-100	18-36, 46-64, 81-99
CYOE_ECOLI	296	7	9	66.64	0.34	0.882	12-29, 38-56, 87-105, 108-128, 133-154, 164-183, 209-229, 233-249, 264-281	10-28, 38-56, 79-97, 108-126, 198-216, 229-247, 269-287
DHG_ECOLI	796	5	6	86.41	0.68	0.913	11-29, 37-58, 63-81, 87-106, 113-130, 707-727	11-37, 41-58, 63-81, 96-110, 119-141
DMSC_ECOLI	287	8	8	91.68	0.81	1.000	10-32, 44-61, 88-107, 117-134, 155-176, 183-201, 223-242, 252-270	10-32, 44-66, 88-107, 113-134, 153-176, 183-203, 223-243, 255-281
DSBB_ECOLI	176	4	3	80.83	0.64	0.866	14-32, 42-63, 145-162	15-31, 50-65, 72-89, 145-162
EDG1_HUMAN	381	7	5	80.67	0.69	0.845	50-71, 122-140, 160-177, 202-221, 257-277	47-71, 79-107, 122-140, 160-185, 202-222, 256-277, 294-314
EGFR_HUMAN	1210	1	1	91.30	0.91	1.000	650-668	646-668
ENVZ_ECOLI	450	2	2	95.12	0.95	1.000	16-35, 163-179	16-35, 162-182
EXBB_ECOLI	244	3	3	88.00	0.76	1.000	25-42, 142-160, 177-195	25-42, 132-150, 178-195
EXBD_ECOLI	141	1	1	100.00	1.00	1.000	26-43	26-43
FCE2_HUMAN	321	1	1	92.31	0.91	1.000	24-45	22-47
FTSH_ECOLI	644	2	2	90.00	0.89	1.000	6-22, 102-120	5-24, 96-120
FTSL_ECOLI	121	1	1	100.00	1.00	1.000	38-57	38-57
FUCP_ECOLI	438	12	11	88.26	0.76	0.957	24-41, 65-85, 92-109, 117-134, 164-181, 212-228, 289-310, 327-345, 352-373, 386-404, 407-428	24-44, 65-85, 92-112, 114-134, 161-181, 214-234, 262-282, 291-311, 327-347, 349-369, 385-405, 408-428
GAA5_HUMAN	462	4	4	94.38	0.93	1.000	260-281, 290-307, 322-341, 431-449	260-281, 286-307, 319-341, 428-449
GLPC_HUMAN	128	1	1	97.92	0.97	1.000	59-81	58-81
GLP_PIG	133	1	1	93.48	0.92	1.000	66-85	63-85
GMCR_HUMAN	400	1	1	88.46	0.87	1.000	327-346	321-346
GPT_CRILO	408	10	8	83.38	0.68	0.949	11-32, 59-76, 94-114, 126-145, 157-179, 222-240, 262-280, 379-397	7-32, 58-79, 95-114, 126-145, 165-184, 195-211, 222-240, 253-269, 275-294, 379-397
GRB_RAT	496	4	2	74.12	0.66	0.707	266-286, 331-350	266-290, 299-316, 331-354, 478-495
HEMA_CDVO	604	1	1	92.86	0.92	1.000	38-55	35-55
HEMA_PI4HA	573	1	1	97.50	0.97	1.000	28-46	28-47
HG2A_HUMAN	296	1	1	88.46	0.87	1.000	53-72	47-72
HISM_SALTY	235	5	3	77.71	0.63	0.775	27-46, 59-79, 203-221	27-47, 59-79, 105-125, 158-178, 200-220
HOXN_ALCEU	351	8	6	69.51	0.41	0.866	20-42, 89-111, 128-149, 230-247, 278-297, 320-338	20-40, 52-72, 95-115, 129-149, 200-220, 244-264, 270-290, 317-337
IG1R_HUMAN	1367	1	1	97.92	0.98	1.000	936-958	936-959
IL2A_HUMAN	272	1	1	100.00	1.00	1.000	241-259	241-259
IL2B_HUMAN	551	1	2	86.19	0.59	0.707	12-30, 247-265	241-265
IM23_SCHMA	218	4	4	92.80	0.88	1.000	14-32, 56-72, 88-107, 184-204	13-36, 56-71, 83-108, 184-205
IMM1_ECOLI	113	3	2	78.02	0.59	0.816	6-24, 86-104	9-25, 39-57, 84-104
IMMA_CITFR	178	4	3	82.40	0.69	0.866	18-35, 106-123, 145-165	14-37, 69-89, 107-123, 143-165
ITA5_MOUSE	1053	1	1	86.54	0.85	1.000	1003-1021	1000-1025
KDPD_ECOLI	894	3	4	97.62	0.97	1.000	403-423, 425-442, 446-465, 478-498	403-444, 446-466, 478-498
KGTP_ECOLI	432	12	11	87.42	0.73	0.957	35-53, 66-84, 96-113, 166-183, 195-214, 244-262, 274-295, 312-330, 340-360, 371-389, 405-423	26-54, 62-80, 96-116, 120-136, 163-185, 196-214, 244-261, 275-301, 312-330, 337-360, 369-392, 403-423
LACY_ECOLI	417	12	11	85.97	0.70	0.957	9-27, 47-66, 76-95, 103-125, 145-164, 167-187, 222-239, 260-283, 291-309, 349-369, 383-400	11-33, 47-67, 75-99, 103-125, 145-163, 168-187, 212-234, 260-281, 291-310, 315-334, 347-366, 380-399
LECI_MOUSE	301	1	1	95.24	0.95	1.000	59-77	59-79
LEP_ECOLI	324	2	2	91.41	0.85	1.000	6-22, 63-81	4-22, 59-77
LSPA_ECOLI	164	4	4	92.57	0.83	1.000	12-31, 69-88, 96-113, 134-153	12-22, 70-88, 96-113, 139-153
MAGL_MOUSE	637	1	1	92.50	0.92	1.000	517-533	517-536
MALF_ECOLI	514	8	8	95.82	0.89	1.000	19-36, 40-58, 72-91, 277-294, 319-336, 371-392, 429-447, 484-504	17-35, 40-58, 73-91, 277-295, 319-337, 371-389, 428-436, 486-504
MALG_ECOLI	296	6	6	85.28	0.71	1.000	18-37, 91-111, 124-144, 160-177, 205-227, 263-281	19-39, 82-102, 124-144, 151-171, 205-225, 260-280
MELB_ECOLI	469	12	11	87.31	0.75	1.000	16-37, 75-93, 102-119, 146-163, 177-194, 232-251, 263-282, 293-314, 320-340, 377-393, 408-426	8-28, 33-53, 76-96, 103-123, 146-166, 172-192, 231-251, 263-283, 293-313, 320-340, 370-390, 408-428
MOTA_ECOLI	295	4	3	76.51	0.57	0.866	8-28, 165-185, 198-220	2-21, 34-51, 171-191, 201-222
MOTB_ECOLI	308	1	1	88.64	0.87	1.000	33-49	28-49
MPRD_HUMAN	277	1	1	88.00	0.86	1.000	191-209	186-210
MTR_ECOLI	414	11	9	78.42	0.56	0.905	13-32, 39-56, 95-114, 129-146, 152-172, 228-246, 284-306, 344-362, 387-407	16-36, 42-62, 89-109, 129-149, 152-172, 191-211, 229-249, 256-276, 286-306, 326-367, 387-407
MYP0_HUMAN	248	1	2	85.65	0.58	0.707	13-33, 156-176	154-179
NEP_HUMAN	749	1	1	89.13	0.88	1.000	32-49	28-50
NGFR_HUMAN	427	1	1	93.18	0.93	1.000	254-272	251-272
OPPB_SALTY	306	6	6	92.17	0.85	1.000	16-37, 103-121, 138-156, 173-190, 229-250, 274-293	10-30, 100-121, 138-158, 173-190, 227-250, 272-293
OPPC_SALTY	302	6	5	88.27	0.80	1.000	38-56, 103-121, 149-171, 218-236, 270-289	38-59, 103-122, 140-160, 164-180, 216-236, 268-290
OPS1_CALVI	371	7	5	79.19	0.64	0.845	50-73, 123-144, 168-186, 218-239, 275-293	48-72, 85-110, 125-144, 164-187, 212-239, 275-298, 306-330
OPSB_HUMAN	348	7	6	84.01	0.72	0.926	38-58, 76-94, 112-130, 153-172, 202-218, 250-270	34-58, 71-96, 111-130, 150-173, 200-227, 250-273, 282-306
PHOR_ECOLI	431	2	1	72.10	0.58	1.000	22-44	14-34, 39-59
PIGR_HUMAN	764	1	1	97.83	0.98	1.000	640-661	639-661
RFBP_SALTY	476	5	5	94.35	0.87	1.000	22-40, 56-72, 90-107, 115-131, 285-303	15-31, 56-72, 90-106, 115-131, 285-301
RHAT_ECOLI	344	10	10	91.67	0.82	1.000	7-26, 40-58, 74-94, 103-121, 136-152, 174-192, 217-234, 259-278, 290-310, 320-338	4-24, 38-58, 74-94, 101-121, 137-157, 175-195, 214-234, 259-279, 290-310, 323-343
RIB1_RAT	605	1	1	100.00	1.00	1.000	438-455	438-455
SECD_ECOLI	615	6	5	83.81	0.76	1.000	10-26, 456-472, 475-494, 499-518, 577-596	10-30, 452-472, 476-497, 504-524, 564-584, 586-605
SECE_ECOLI	127	3	3	98.21	0.97	1.000	19-36, 45-62, 94-111	19-36, 45-63, 93-111
SECY_ECOLI	443	10	10	91.33	0.83	1.000	23-41, 77-98, 122-140, 154-174, 189-207, 217-237, 274-294, 318-337, 369-387, 397-414	23-42, 75-95, 122-139, 154-174, 183-203, 217-237, 274-294, 316-335, 376-395, 399-414
SSRG_RAT	185	4	3	83.80	0.72	0.866	30-48, 59-77, 136-157	28-48, 55-76, 136-157, 164-184
SUIS_HUMAN	1827	1	2	97.00	0.69	0.707	13-31, 622-639	13-32
TAL6_HUMAN	202	4	4	93.16	0.88	1.000	10-30, 46-68, 94-113, 162-179	10-30, 46-70, 89-116, 162-182
TCB1_RABIT	319	1	1	93.18	0.92	1.000	293-311	292-313
TCR1_ECOLI	399	11	12	92.28	0.84	1.000	7-28, 47-66, 74-93, 104-121, 133-154, 163-181, 215-236, 248-268, 279-297, 300-320, 340-361, 368-385	8-28, 44-64, 76-96, 104-124, 133-153, 161-181, 216-236, 246-267, 279-320, 338-358, 365-385
TOLR_ECOLI	142	1	1	84.00	0.80	1.000	24-40	16-40
TRBM_HUMAN	575	1	1	91.67	0.91	1.000	517-536	516-539
TRSR_HUMAN	760	1	2	81.40	0.54	0.707	71-87, 540-558	63-88
UHPT_ECOLI	463	12	11	90.81	0.82	0.957	27-45, 56-76, 97-116, 119-136, 160-181, 191-210, 260-278, 300-318, 327-344, 357-379, 426-446	28-45, 56-80, 97-113, 120-136, 159-183, 191-210, 260-278, 301-318, 327-344, 352-376, 388-406, 422-446
UPKB_BOVIN	259	4	4	95.21	0.92	1.000	12-30, 59-80, 86-106, 229-248	12-37, 60-80, 86-106, 229-249
VMT2_IAANN	97	1	1	96.59	0.93	1.000	26-43	25-42
VNB_INBBE	99	1	1	95.45	0.94	1.000	21-40	19-40
Average				88.83	0.80	0.954