ID GAA5_HUMAN STANDARD; PRT; 462 AA.
AC P31644;
DT 01-JUL-1993 (REL. 26, CREATED)
DT 01-JUL-1993 (REL. 26, LAST SEQUENCE UPDATE)
DT 01-JUN-1994 (REL. 29, LAST ANNOTATION UPDATE)
DE GAMMA-AMINOBUTYRIC-ACID RECEPTOR ALPHA-5 SUBUNIT PRECURSOR (GABA(A)
DE RECEPTOR).
GN GABRA5.
OS HOMO SAPIENS (HUMAN).
OC EUKARYOTA; METAZOA; CHORDATA; VERTEBRATA; TETRAPODA; MAMMALIA;
OC EUTHERIA; PRIMATES.
RN [1]
RN [2]
RP SEQUENCE FROM N.A.
RP SEQUENCE FROM N.A.
RX MEDLINE; 93271965.
RX MEDLINE; 92339652.
RA KNOLL J.H., SINNETT D., WAGSTAFF J., GLATT K.A., WILCOX A.S.,
RA WHITING P.J., WINGROVE P., SIKELA J.M., LALANDE M.;
RA WINGROVE P., HADINGHAM K., WAFFORD K., KEMP J.A., RAGAN C.I.,
RA WHITING P.;
RL HUM. MOL. GENET. 2:183-189(1993).
RL BIOCHEM. SOC. TRANS. 20:18S-18S(1992).
CC -!- FUNCTION: GABA, THE MAJOR INHIBITORY NEUROTRANSMITTER IN THE
CC VERTEBRATE BRAIN, MEDIATES NEURONAL INHIBITION BY BINDING TO THE
CC GABA/BENZODIAZEPINE RECEPTOR AND OPENING AN INTEGRAL CHLORIDE
CC CHANNEL.
CC -!- SUBUNIT: GENERALLY PENTAMERIC. THERE ARE FIVE TYPES OF GABA(A)
CC RECEPTOR CHAINS: ALPHA, BETA, GAMMA, DELTA, AND RHO.
CC -!- SUBCELLULAR LOCATION: INTEGRAL MEMBRANE PROTEIN.
CC -!- SIMILARITY: BELONGS TO THE LIGAND-GATED IONIC CHANNELS FAMILY.
DR EMBL; L08485; G182916; -.
DR EMBL; A28104; E204336; -.
DR PIR; A48933; A48933.
DR MIM; 137142; -.
DR PROSITE; PS00236; NEUROTR_ION_CHANNEL; 1.
KW POSTSYNAPTIC MEMBRANE; IONIC CHANNEL; GLYCOPROTEIN; SIGNAL;
KW TRANSMEMBRANE; MULTIGENE FAMILY.
FT SIGNAL 1 31 POTENTIAL.
FT CHAIN 32 462 GABA(A) RECEPTOR ALPHA-5 SUBUNIT.
FT DOMAIN 32 259 EXTRACELLULAR (PROBABLE).
FT TRANSMEM 260 281 PROBABLE.
FT TRANSMEM 286 307 PROBABLE.
FT TRANSMEM 319 341 PROBABLE.
FT DOMAIN 342 427 CYTOPLASMIC (PROBABLE).
FT TRANSMEM 428 449 PROBABLE.
FT CARBOHYD 45 45 POTENTIAL.
FT CARBOHYD 145 145 POTENTIAL.
FT CARBOHYD 207 207 POTENTIAL.
FT CARBOHYD 236 236 POTENTIAL.
FT DISULFID 173 187 BY SIMILARITY.
SQ SEQUENCE 462 AA; 52146 MW; 4424DE2C CRC32;
MDNGMFSGFI MIKNLLLFCI SMNLSSHFGF SQMPTSSVKD ETNDNITIFT RILDGLLDGY
DNRLRPGLGE RITQVRTDIY VTSFGPVSDT EMEYTIDVFF RQSWKDERLR FKGPMQRLPL
NNLLASKIWT PDTFFHNGKK SIAHNMTTPN KLLRLEDDGT LLYTMRLTIS AECPMQLEDF
PMDAHACPLK FGSYAYPNSE VVYVWTNGST KSVVVAEDGS RLNQYHLMGQ TVGTENISTS
TGEYTIMTAH FHLKRKIGYF VIQTYLPCIM TVILSQVSFW LNRESVPART VFGVTTVLTM
TTLSISARNS LPKVAYATAM DWFIAVCYAF VFSALIEFAT VNYFTKRGWA WDGKKALEAA
KIKKKREVIL NKSTNAFTTG KMSHPPNIPK EQTPAGTSNT TSVSVKPSEE KTSESKKTYN
SISKIDKMSR IVFPVLFGTF NLVYWATYLN REPVIKGAAS PK
//
1 2 3 4 5 6
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. | . | . | . | . | . |
0000 MDNGMFSGFIMIKNLLLFCISMNLSSHFGFSQMPTSSVKDETNDNITIFTRILDGLLDGY
0060 DNRLRPGLGERITQVRTDIYVTSFGPVSDTEMEYTIDVFFRQSWKDERLRFKGPMQRLPL
0120 NNLLASKIWTPDTFFHNGKKSIAHNMTTPNKLLRLEDDGTLLYTMRLTISAECPMQLEDF
0180 PMDAHACPLKFGSYAYPNSEVVYVWTNGSTKSVVVAEDGSRLNQYHLMGQTVGTENISTS
______________________ _______________
0240 TGEYTIMTAHFHLKRKIGYFVIQTYLPCIMTVILSQVSFWLNRESVPARTVFGVTTVLTM
_______ _______________________
0300 TTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKRGWAWDGKKALEAA
0360 KIKKKREVILNKSTNAFTTGKMSHPPNIPKEQTPAGTSNTTSVSVKPSEEKTSESKKTYN
______________________
0420 SISKIDKMSRIVFPVLFGTFNLVYWATYLNREPVIKGAASPK
(predicted transmembrane regions are indicated in blue)
(observed transmembrane regions are overlined in red)
Rating of the prediction against the observed structure
Agreement factor (Q) = 94.38%
Correlation coefficient (C) = 0.93
Ratio of segment matches = 1
Detail of predicted transmembrane segments:
Number of predicted transmembrane segments: 4
Number of observed transmembrane segments: 4
predicted observed
#TM begin end begin end
1 260 281 260 281
2 290 307 286 307
3 322 341 319 341
4 431 449 428 449